KMID : 0380020040190050363
|
|
Korean Journal of Biotechnology and Bioengineering 2004 Volume.19 No. 5 p.363 ~ p.367
|
|
Purification and Characterization Sucrose phosphorylase in Leuconostoc mesenteroides NRRL B-1149
|
|
Lee Jin-Ha
Park Jun-Sung Park Hyun-Jung Cho Jae-Young Choi Jung-Sik Kim Do-Man
|
|
Abstract
|
|
|
Leuconostoc mesenteroides NRRL B-1149 produces various glucoseyltransferases for the synthesis of dextran, levan and glucose-1-phosphate using sucrose as a substrate. A sucrose phosphorylase (1149SPase) was purified from L. mesenteroides NRRL B-1149 culture by using hollow fiber filtration (30 kDa cut off), Toyopearl DEAE 650 M column chromatography and following two times of DEAE-Sepharose column chromatographies. The specific activity of the purified 1149SPase was 25.7 (U/mg) with yield. The 1149SPase showed a molecular size of 56 kDa on denatured SDS-PAGE. The N-terminal amino acid sequence of the enzyme was MEIQNKAM. The optimum pH and temperature of this enzyme were 6.2~6.5 and 37^{circ}C, respectively. It had an apparent K_{m} of 6.0 mM and K_{cat} of 1.62/s for sucrose. 1149SPase crystal was formed by hanging drop diffusion technique using 20 mM calcium chloride dihydrate, 100 mM sodium acetate trihydrate pH 4.6 and 2-methyl-2,4-pentanediol as vaporizing and reservation solution. The 1149SPase catalyzes transferring of glucose from isomaltose or sucrose to salicin and salicyl alcohol by disproportionation reaction or acceptor reaction and synthesized two acceptor products, respectively.
|
|
KEYWORD
|
|
Leuconostoc mesenteroides, sucrose phosphorylase, purification, crystal, acceptor reaction, disproportionation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|